With a focus on several recent single-molecule fluorescence microscopy studies on DNA replication and repair in living bacteria cells, Yilai’s Current Opinion in Microbiology review discusses how  the development of single-molecule microscopy has provided a new perspective on these fundamental processes.

Y. Li, J.W. Schroeder, L.A. Simmons, and J.S. Biteen, “Visualizing bacterial DNA replication and repair with molecular resolution,” Current Opinion in Microbiology 43 38-45 (2018).  DOI: 10.1016/j.mib.2017.11.009

Congratulations to Hannah who advanced to candidacy! Hannah is now the first Program in Chemical Biology PhD candidate in the Biteen Lab.

With a combination of biophysical, biochemical, and biological approaches, Hannah Tuson and Matt Foley have identified in the gut bacterium Bacteroides thetaiotaomicron a new paradigm for protein complex formation in bacteria: binding proteins form immobile complexes that transiently associate with a mobile enzyme partner.

H.H. Tuson, M.H. Foley, N.M. Koropatkin and J.S. Biteen, “The Starch Utilization System Assembles around Stationary Starch-Binding Proteins,” Biophysical Journal in press (2018).
https://doi.org/10.1016/j.bpj.2017.12.015

Press Release:
http://ns.umich.edu/new/releases/25399-got-starch-there-s-bacteria-in-your-gut-for-that

The Biteen Lab is excited to welcome Brianna and Karen to the lab for rotations this winter!

Plasmonic nanoparticles can make dyes brighter, but this coupling also introduces a mislocalization between the measured dye position and where it actually is. Here, we measure this “scooching” effect on the nanometer scale!

Full article: B. Fu, B.P. Isaacoff, and J.S. Biteen, “Super-Resolving the Actual Position of Single Fluorescent Molecules Coupled to a Plasmonic Nanoantenna,” ACS Nano (2017).

Press release here

Hannah has been selected as a 2017 Department of Education Graduate Assistance in Areas of National Need (GAANN) Fellow!

Congrats to Hannah Chia on winning a PPG poster award for her outstanding presentation at this summer’s Karle Symposium!

The thematic issue on Super-Resolution and Single-Molecule Imaging, edited by Katherine Willets and Julie Biteen has now been published in Chemical Reviews. Check out a great set of reviews that outline the state-of-the-art in this field!

Chemical Reviews 117 (11), pages 7241-7614 (June 14, 2017).

The Biteen Lab welcomes Nhat Hoang Van Le, an incoming Chemistry graduate student who will be rotating in our lab this summer!

The Biteen Lab welcomes Katie Perrotta, a rising senior at Muhlenberg College, who will be working with us this summer as part of the University of Michigan Chemistry REU program!