Previously, I have worked in the laboratory of Dr. David Boehr (Penn State University) on implementing and developing latest methyl-detected NMR experiments for studying allosteric networks of RNA dependent RNA polymerase (RdRp).
At Penn State University I worked with Dr. Scott Showalter on various projects ranging from computational MD simulations on RNA, micro-RNA structure mapping, to NMR based protein structure determination of well folded proteins and intrinsically disordered proteins. I also helped develop the carbon-detect NMR experiments for quick and efficient assignments of proteins.
During my graduate work, I was introduced to my Ph.D. advisor Dr. Junji Iwahara (UTMB), an expert in the field of NMR spectroscopy. I learned the inner workings of NMR instruments and the principles of NMR from my mentor and pursued to write NMR pulse sequences. In addition to NMR, I learned various computational methods including MATLAB, molecular biology, protein expression & purification techniques and fluorescence based stopped flow kinetics. I had successfully applied these techniques, mainly NMR to study kinetics of processes involving DNA binding proteins.
I pursued my bachelors in Industrial Biotechnology at Anna University where I was exposed to various aspects of Biotechnology from Immunology, Bioprocess Engineering, Microbiology to Computational Biology. I was intrigued by proteins and bio-physical techniques to analyze them. I worked with Dr. Gautam Pennathur (Anna University) to work on Bio-fuel cells and with Dr. B. Jayaram (IIT Delhi) to work on ab-initio protein folding problem.