Western Lake Erie Time Series

Overview

The Western Basin of Lake Erie experiences cyanobacterial harmful algal blooms (cyanoHABs) every year between May through October. These events produce mass amounts of toxic cyanobacterial mass, threatening both human and environmental health. We began working with NOAA Great Lakes Environmental Research Laboratory (GLERL) and the Cooperative Institute for Great Lakes Research (CIGLR) to sample Western Lake Erie during pre-bloom, bloom, and post-bloom conditions for genomic, metabolomic, and physiochemical data.

In the Geomicrobiology laboratory, we utilize high throughput, multi-omic techniques to identify the organisms, genes and their transcript levels, and toxic metabolites produced within each collected sample. By collecting these powerful datasets over a spatiotemporally resolved time series that represents seasonal and interannual variation, we hope to better understand how and why cyanoHABs in the Great Lakes are changing. These data are bioinformatically computed and deposited in the Great Lakes Atlas for Multi-Omics Research (GLAMR), along with all other publicly available multi-omic data sets from the Great Lakes.

Current Team

Anders Kiledal

Postdoc

kiledal@umich.edu

Lauren Hart

PhD student

lnhart@umich.edu

Data

Data from 2014-2021 are available.

Sequences have been deposited in the NCBI database under the BioProject accession number PRJNA702128. Metagenomic and metatranscriptomic raw reads can be found in the NCBI database under the BioProject accession numbers PRJNA464361 and PRJNA370007, respectively.

Publications

  1. Yancey, C. E., Yu, F., Tripathi, A., Sherman, D. H., & Dick, G. J. (2023). Expression of Microcystis biosynthetic gene clusters in natural populations suggests temporally dynamic synthesis of novel and known secondary metabolites in western Lake Erie. Applied and Environmental Microbiology, e02092-22.
  2. Yancey, C. E., Smith, D. J., Den Uyl, P. A., Mohamed, O. G., Yu, F., Ruberg, S. A., … & Dick, G. J. (2022). Metagenomic and metatranscriptomic insights into population diversity of Microcystis blooms: spatial and temporal dynamics of mcy genotypes, including a partial operon that can be abundant and expressed. Applied and Environmental Microbiology88(9), e02464-21.
  3. Kharbush, J. J., Smith, D. J., Powers, M., Vanderploeg, H. A., Fanslow, D., Robinson, R. S., … & Pearson, A. (2019). Chlorophyll nitrogen isotope values track shifts between cyanobacteria and eukaryotic algae in a natural phytoplankton community in Lake Erie. Organic geochemistry128, 71-77.

Funding

  1. NIH and NSF awards to the Great Lakes Center for Fresh Waters and Human Health (NIH award 1P01ES028939-01 and NSF award OCE-1840715) and the Cooperative Institute of Great Lakes Research (NA17OAR4320152). This CIGLR contribution number is 1191.
  2. NOAA ECOHAB award (NA17NOS4780186)
  3. Support was also provided by the NOAA OAR ‘Omics and NOAA OAR Ocean Technology Development Initiative
  4. NSF OCE-1736629