Dr. Jakob's research is on the biochemical aspects of the bacterial response to heat shock.
Dr. Jakob received her B.S. degree in 1991 from Regensburg University in Germany, and her Ph.D. in 1995 also from Regensburg University. From 1996 until 1998 she was a postdoctoral Research Fellow at the University of Michigan with a fellowship from the German government, and from 1998 until 2001 she was an Assistant Research scientist at UM.
Dr. Jakob has been chosen as a "Biological Scholar" at the University of Michigan, a prestigious designation awarded by a University-wide committee. She is also a recipient of the Burroughs Wellcome Fund Career Award in the Biomedical Sciences 2000. In addition, she won a Sokol Postdoctoral Award in 1998.
Post-Doctoral and Graduate Students
Ellen received her PhD in 2017 at the University of Washington where she worked on interventions for cardiac dysfunction in aging. Her work in the Jakob lab will build on that aging background, looking at polyphosphate abundance and localization in the aging brain and how this may correlate with histological signatures of Alzheimer’s disease in both mouse and human tissue.
Daphne Bazopoulou joined the Jakob Lab in 2014 as a postdoctoral fellow. She is utilizing sorting techniques, oxidative stress assays and gene expression profiling methods to investigate how developmental ROS contributes to aging and lifespan determination in C. elegans.
Office | 4041 Nat. Sci.
Jan obtained his PhD from the University of Potsdam in 2013, where he worked in the group of Prof. Dr. Silke Leimkuehler. He joined the Jakob lab as postdoctoral fellow in 2014 and identified HdeB as effective chaperone under acid stress in vivo and in vitro. Jan has focused on understanding the physiological consequences for bacteria when exposed to protein-damaging oxidants such as hypochlorous acid, which is the active antimicrobial component of bleach. In his spare time, Jan enjoys going to the gym, spends time with his friends and discovers his favourite city Detroit in more detail.
Bastian joined the Jakob lab in October 2012 for his Ph.D. to investigate the binding site of the redox regulated chaperone Hsp33. He utilizes techniques such as the incorporation of unnatural amino acids for NMR as well as crosslinking studies to determine the regions involved in preventing substrates from aggregation.
Office | 4041 Nat. Sci.
Daniela joined the Jakob lab in 2007 to work on her PhD thesis investigating the role of oxidative stress in aging in Caenorhabditis elegans. After obtaining her PhD in 2012, she stayed on as a Postdoc in the Jakob lab. Daniela's research interests are in identifying why certain cancer cells become resistant to chemotherapy and how to restore drug sensitivity. She primarily uses cell-based assays, fluorescence microscopy and flow cytometry assays to study cisplatin resistance in mammalian cancer cell lines.
Wilhelm joined the Jakob lab in February 2012 for his Master Thesis and stayed as a PhD student to investigate how eukaryotes (mainly yeast) deal with oxidative stress. He discovered that the highly conserved ATPase Get3, an essential protein in mammals, functions as a redox-sensitive chaperone in eukaryotes. Utilizing spectroscopic techniques, mass spectrometric analysis and EM microscopy Wilhelm and colleagues showed that Get3 undergoes crucial changes in its structure and oligomerization state upon oxidation to become an effective chaperone holdase. In his spare time Wilhelm enjoys to work out in the gym, go for a run and to read and travel
Kathrin received her PhD in 2017 at Heidelberg University where she worked on stress-induced protein S-thiolation in African trypanosomes. She joined the Jakob lab in October 2017 to follow up on the functional characterization of the redox-regulated Get3 chaperone activity and study this thiol-based switching mechanism also in the highly conserved mammalian homolog TRC40.
Justine has started her PhD in the Jakob lab in April 2016. Her research focuses on the role of polyphosphate in amyloidogenic diseases including Alzheimer's disease and Parkinson's diseases. In vitro she is characterizing the underlying mechanisms of the interaction between polyphosphate and its amyloidogenic client proteins. Within a neuronal cell line she is investigating these findings in the context of a cellular system and tries to further unravel the protective role that polyphosphate has against amyloid toxicity.
Chris joined the laboratory in December 2016, acting as both lab manager and part-time researcher; his current projects revolve around adapting the tripartite fusion system into mammalian lines, to investigate protein folding in higher order species. He obtained his B.S. in Biochemistry from Michigan State University, and M.S. in Genetics from Wayne State University. This position allows him to combine his interests in management and student support with his background in molecular biology. Outside of the lab, he enjoys cooking, baking, beer, camping, and taking care of too many cats.
Research Laboratory Technician Senior
Office | 4007 Nat. Sci.
Ken has worked as a lab technician at U of M for nearly ten years. He joined the Bardwell lab from an HHMI lab in June 2013. Ken’s work is mainly on protein expression and purification. His work consists of making all kinds of constructs for E. coli, Baculovirus insect cell and yeast systems as well as purifying proteins with or without tags using the AKTA system. He enjoys screening and optimizing the protein crystals as well. Ken likes traveling, swimming and fishing.