Publications – Jakob Lab

Targeted protein degradation: from small molecules to complex organelles-a Keystone Symposia report. Cable J, Weber-Ban E, Clausen T, Walters KJ, Sharon M, Finley DJ, Gu Y, Hanna J, Feng Y, Martens S, Simonsen A, Hansen M, Zhang H, Goodwin JM, Reggio A, Chang C, Ge L, Schulman BA, Deshaies RJ, Dikic I, Harper JW, Wertz IE, Thomä NH, Słabicki M, Frydman J, Jakob U, David DC, Bennett EJ, Bertozzi CR, Sardana R, Eapen VV, Carra S . Ann N Y Acad Sci. 2022 Jan 8. doi: 10.1111/nyas.14745. Online ahead of print. PMID:35000205

Polyphosphate drives bacterial heterochromatin formation. Beaufay F, Amemiya HM, Guan J, Basalla J, Meinen BA, Chen Z, Mitra R, Bardwell JCA, Biteen JS, Vecchiarelli AG, Freddolino PL, Jakob U. Sci Adv. 2021 Dec 24;7(52):eabk0233. doi: 10.1126/sciadv.abk0233. Epub 2021 Dec 22. PMID: 34936433

Shaping longevity early in life: developmental ROS and H3K4me3 set the clock. Oleson BJ, Bazopoulou D, Jakob U. Cell Cycle. 2021 Nov;20(22):2337-2347. doi: 10.1080/15384101.2021.1986317. Epub 2021 Oct 17. PMID: 34657571

Depletion of mitochondrial inorganic polyphosphate (polyP) in mammalian cells causes metabolic shift from oxidative phosphorylation to glycolysis. Solesio ME, Xie L, McIntyre B, Ellenberger M, Mitaishvili E, Bhadra-Lobo S, Bettcher LF, Bazil JN, Raftery D, Jakob U, Pavlov EV .Biochem J. 2021 Apr 30;478(8):1631-1646. doi: 10.1042/BCJ20200975. PMID: 33843973

Automated phenotyping and lifespan assessment of a C. elegans model of Parkinson’s disease. Kim M, Knoefler D, Quarles E, Jakob U, Bazopoulou D. Transl Med Aging. 2020;4:38-44. doi: 10.1016/j.tma.2020.04.001. Epub 2020 Apr 19 PMID: 33569522

Induction of the reactive chlorine-responsive transcription factor RclR in Escherichia coli following ingestion by neutrophils. Königstorfer A, Ashby LV, Bollar GE, Billiot CE, Gray MJ, Jakob U, Hampton MB, Winterbourn CC. Pathog Dis. 2021 Jan 9;79(1):ftaa079. doi: 10.1093/femspd/ftaa079. PMID: 33351093

Thiol-based switching mechanisms of stress-sensing chaperones. Ulrich K, Schwappach B, Jakob U. Biol Chem. 2020 Oct 5;402(3):239-252. doi: 10.1515/hsz-2020-0262. Print 2021 Feb 23. PMID: 32990643 Review.

Mycobacterium tuberculosis Rv0991c Is a Redox-Regulated Molecular Chaperone. Becker SH, Ulrich K, Dhabaria A, Ueberheide B, Beavers W, Skaar EP, Iyer LM, Aravind L, Jakob U, Darwin KH. mBio. 2020 Aug 25;11(4):e01545-20. doi: 10.1128/mBio.01545-20. PMID: 32843553

Polyphosphate Functions In Vivo as an Iron Chelator and Fenton Reaction Inhibitor. Beaufay F, Quarles E, Franz A, Katamanin O, Wholey WY, Jakob U. mBio. 2020 Jul 28;11(4):e01017-20. doi: 10.1128/mBio.01017-20. PMID: 32723918

Accumulation of Nucleolar Inorganic Polyphosphate Is a Cellular Response to Cisplatin-Induced Apoptosis. Xie L, Rajpurkar A, Quarles E, Taube N, Rai AS, Erba J, Sliwinski B, Markowitz M, Jakob U, Knoefler D. Front Oncol. 2019 Dec 12;9:1410. doi: 10.3389/fonc.2019.01410. eCollection 2019. PMID: 31921667

Developmental ROS individualizes organismal stress resistance and lifespan. Bazopoulou D, Knoefler D, Zheng Y, Ulrich K, Oleson BJ, Xie L, Kim M, Kaufmann A, Lee YT, Dou Y, Chen Y, Quan S, Jakob U. Nature. 2019 Dec;576(7786):301-305. doi: 10.1038/s41586-019-1814-y. Epub 2019 Dec 4. PMID: 31801997

The Anti-Aggregation Holdase Hsp33 Promotes the Formation of Folded Protein Structures. Moayed F, Bezrukavnikov S, Naqvi MM, Groitl B, Cremers CM, Kramer G, Ghosh K, Jakob U, Tans SJ. Biophys J. 2020 Jan 7;118(1):85-95. doi: 10.1016/j.bpj.2019.10.040. Epub 2019 Nov 11. PMID: 31757359

An essential thioredoxin-type protein of Trypanosoma brucei acts as redox-regulated mitochondrial chaperone. Currier RB, Ulrich K, Leroux AE, Dirdjaja N, Deambrosi M, Bonilla M, Ahmed YL, Adrian L, Antelmann H, Jakob U, Comini MA, Krauth-Siegel RL. PLoS Pathog. 2019 Sep 26;15(9):e1008065. doi: 10.1371/journal.ppat.1008065. eCollection 2019 Sep. PMID: 31557263

Mechanistic insights into the protective roles of polyphosphate against amyloid cytotoxicity. Lempart J, Tse E, Lauer JA, Ivanova MI, Sutter A, Yoo N, Huettemann P, Southworth D, Jakob U. Life Sci Alliance. 2019 Sep 18;2(5):e201900486. doi: 10.26508/lsa.201900486. Print 2019 Oct. PMID: 31533964

Polyphosphate Initiates Tau Aggregation through Intra- and Intermolecular Scaffolding. Wickramasinghe SP, Lempart J, Merens HE, Murphy J, Huettemann P, Jakob U, Rhoades E.Biophys J. 2019 Aug 20;117(4):717-728. doi: 10.1016/j.bpj.2019.07.028. Epub 2019 Jul 24. PMID: 31400913

The molecular chaperone Hsp33 is activated by atmospheric-pressure plasma protecting proteins from aggregation. Krewing M, Stepanek JJ, Cremers C, Lackmann JW, Schubert B, Müller A, Awakowicz P, Leichert LIO, Jakob U, Bandow JE.J R Soc Interface. 2019 Jun 28;16(155):20180966. doi: 10.1098/rsif.2018.0966. Epub 2019 Jun 19. PMID: 31213177

The role of thiols in antioxidant systems. Ulrich K, Jakob U. Free Radic Biol Med. 2019 Aug 20;140:14-27. doi: 10.1016/j.freeradbiomed.2019.05.035. Epub 2019 Jun 13. PMID: 31201851 Review.

Chaperone activation and client binding of a 2-cysteine peroxiredoxin. Teixeira F, Tse E, Castro H, Makepeace KAT, Meinen BA, Borchers CH, Poole LB, Bardwell JC, Tomás AM, Southworth DR, Jakob U. Nat Commun. 2019 Feb 8;10(1):659. doi: 10.1038/s41467-019-08565-8. PMID: 30737390

Role of Polyphosphate in Amyloidogenic Processes. Lempart J, Jakob U. Cold Spring Harb Perspect Biol. 2019 May 1;11(5):a034041. doi: 10.1101/cshperspect.a034041. PMID: 30617049 Review.

Inorganic polyphosphate, a multifunctional polyanionic protein scaffold. Xie L, Jakob U. J Biol Chem. 2019 Feb 8;294(6):2180-2190. doi: 10.1074/jbc.REV118.002808. Epub 2018 Nov 13. PMID: 30425096 Review.

Extraction and Quantification of Polyphosphate (polyP) from Gram-negative Bacteria. Dahl JU, Xie L, Jakob U. Bio Protoc. 2018 Sep 20;8(18):e3011. doi: 10.21769/BioProtoc.3011. eCollection 2018 Sep 20. PMID: 34395801

Polyphosphate Stabilizes Protein Unfolding Intermediates as Soluble Amyloid-like Oligomers. Yoo NG, Dogra S, Meinen BA, Tse E, Haefliger J, Southworth DR, Gray MJ, Dahl JU, Jakob U. J Mol Biol. 2018 Oct 19;430(21):4195-4208. doi: 10.1016/j.jmb.2018.08.016. Epub 2018 Aug 18. PMID: 30130556

Phase shifts in protein folding space: links to stress adaptation and disease. Alberti S, Jakob U. Mol Biol Cell. 2018 Mar 15;29(6):695. doi: 10.1091/mbc.E17-11-0685. PMID: 29535178

Maintaining a Healthy Proteome during Oxidative Stress. Reichmann D, Voth W, Jakob U. Mol Cell. 2018 Jan 18;69(2):203-213. PMID: 29351842

Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? Henderson D, Huebner C, Markowitz M, Taube N, Harvanek ZM, Jakob U, Knoefler D. Redox Biol. 2018 Apr;14:386-390. PMID: 29055282

Stress-Activated Chaperones: A First Line of Defense. Voth W, Jakob U. Trends Biochem Sci. 2017 Nov;42(11):899-913.
PMID: 28893460

Pseudomonas aeruginosa defense systems against microbicidal oxidants. Groitl B, Dahl JU, Schroeder JW, Jakob U. Mol Microbiol. 2017 Nov;106(3):335-350. PMID: 28795780

The anti-inflammatory drug mesalamine targets bacterial polyphosphate accumulation. Dahl JU, Gray MJ, Bazopoulou D, Beaufay F, Lempart J, Koenigsknecht MJ, Wang Y, Baker JR, Hasler WL, Young VB, Sun D, Jakob U. Nat Microbiol. 2017 Jan 23;2:16267. PMID: 28112760

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo. Dahl JU, Koldewey P, Bardwell JC, Jakob U. J Vis Exp. 2016 Oct 23;(116). PMID: 27805614

Polyphosphate: A Conserved Modifier of Amyloidogenic Processes. Cremers CM, Knoefler D, Gates S, Martin N, Dahl JU, Lempart J, Xie L, Chapman MR, Galvan V, Southworth DR, Jakob U. Mol Cell. 2016 Sep 1;63(5):768-80. PMID: 27570072.

Do Nucleic Acids Moonlight as Molecular Chaperones? Docter BE, Horowitz S, Gray MJ, Jakob U, and Bardwell JCA. Nucleic Acids Research. 2016 Apr 21. PMID: 27105849

Protein unfolding as a switch from self-recognition to high-affinity client binding. Groitl B, Horowitz S, Makepeace KA, Petrotchenko EV, Borchers CH, Reichmann D, Bardwell JC, Jakob U. Nat Commun. 2016 Jan 20;7:10357. doi: 10.1038/ncomms10357. PMID: 26787517 Related citations.

HdeB functions as an acid-protective chaperone in bacteria. Dahl JU, Koldewey P, Salmon L, Horowitz S, Bardwell JC, Jakob U. J Biol Chem. 2015 Apr 17;290(16):9950. PMID: 25888567 Related citations.

Does the transcription factor NemR use a regulatory sulfenamide bond to sense bleach?
Gray MJ, Li Y, Leichert LI, Xu Z, Jakob U. Antioxid Redox Signal. 2015 Apr 13. [Epub ahead of print]. PMID: 25867078 Related citations.

Protein quality control under oxidative stress conditions. Dahl JU, Gray MJ, Jakob U. J Mol Biol. 2015 Apr 10;427(7):1549-63. doi: 10.1016/j.jmb.2015.02.014. Epub 2015 Feb 16. Review. PMID: 25698115 Related citations

Mitochondrial peroxiredoxin functions as crucial chaperone reservoir in Leishmania infantum.
Teixeira F, Castro H, Cruz T, Tse E, Koldewey P, Southworth DR, Tomás AM, Jakob U. Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E616-24. doi: 10.1073/pnas.1419682112. Epub 2015 Feb 2. PMID: 25646478 Related citations

Oxidative stress protection by polyphosphate-new roles for an old player.
Gray MJ, Jakob U. Curr Opin Microbiol. 2015 Apr;24:1-6. doi: 10.1016/j.mib.2014.12.004. Epub 2015 Jan 10. Review. PMID: 25589044 Related citations

About the dangers, costs and benefits of living an aerobic lifestyle.
Knoefler D, Leichert LI, Thamsen M, Cremers CM, Reichmann D, Gray MJ, Wholey WY, Jakob U.
Biochem Soc Trans. 2014 Aug;42(4):917-21. doi: 10.1042/BST20140108. PMID: 25109979 Related citations

HdeB Functions as an Acid-Protective Chaperone in Bacteria. Dahl JU, Koldewey P, Salmon L, Horowitz S, Bardwell JC, Jakob U. J Biol Chem. 2014 Nov 12. pii: jbc.M114.612986. [Epub ahead of print]
PMID: 25391835 Free Article Related citations

The ‘mitoflash’ probe cpYFP does not respond to superoxide. Schwarzländer M, Wagner S, Ermakova YG, Belousov VV, Radi R, Beckman JS, Buettner GR, Demaurex N, Duchen MR, Forman HJ, Fricker MD, Gems D, Halestrap AP, Halliwell B, Jakob U, Johnston IG, Jones NS, Logan DC, Morgan B, Müller FL, Nicholls DG, Remington SJ, Schumacker PT, Winterbourn CC, Sweetlove LJ, Meyer AJ, Dick TP, Murphy MP. Nature. 2014 Oct 23;514(7523):E12-4. doi: 10.1038/nature13858. No abstract available. PMID: 25341790

The Protein Targeting Factor Get3 Functions as ATP-Independent Chaperone under Oxidative Stress Conditions
Wilhelm Voth,Markus Schick, Stephanie Gates, Sheng Li, Fabio Vilardi, Irina Gostimskaya, Daniel R. Southworth, Blanche Schwappach, and Ursula Jakob Molecular Cell, 2014 Oct 2, 56, 1–12. Epub 2014 Sept 18. PMCID pending. (Abstract)

Bile salts act as effective protein-unfolding agents and instigators of disulfide stress in vivo.
Cremers CM1, Knoefler D, Vitvitsky V, Banerjee R, Jakob U.
Proc Natl Acad Sci U S A. 2014 Apr 4. [Epub ahead of print] PMID:24706920. (Abstract)

Thiol-Based Redox Switches. Groitl B, Jakob U. Epub 2014 Mar 18. Biochim Biophys Acta. [Epub ahead of print] Review. PMID:24657586. (Abstract)

Polyphosphate is a primordial chaperone. Gray MJ, Wholey WY, Wagner NO, Cremers CM, Mueller-Schickert A, Hock NT, Krieger AG, Smith EM, Bender RA, Bardwell JC, Jakob U. Epub 2014 Feb 20.
Mol Cell. 2014 Mar 6;53(5):689-99. PMID:24560923. (Abstract)

Conditionally and Transiently Disordered Proteins: Awakening Cryptic Disorder To Regulate Protein Function.
Jakob U, Kriwacki R, Uversky VN. Epub . 2014 Feb 6.
Chem Rev. 2014 Feb 6. [Epub ahead of print]. PMID:24502763. (Abstract)

The RclR protein is a reactive chlorine-specific transcription factor in Escherichia coli.
Parker BW, Schwessinger EA, Jakob U, Gray MJ. Epub 2013 Sep 27.
J Biol Chem. 2013 Nov 8;288(45):32574-84. doi: 10.1074/jbc.M113.503516. PMCID: PMC3820890. (Abstract)

Oxidant sensing by reversible disulfide bond formation. Cremers CM, Jakob U. Epub 2013 Jul 16.
J Biol Chem. 2013 Sep 13;288(37):26489-96. doi: 10.1074/jbc.R113.462929. Review. PMCID: PMC3772196. (Abstract)

Bacterial responses to reactive chlorine species. Gray MJ, Wholey WY, Jakob U. Epub 2013 Jun 14.
Annu Rev Microbiol. 2013;67:141-60. doi: 10.1146/annurev-micro-102912-142520. PMCID:PMC3891400. (Abstract)

Nonnative disulfide bond formation activates the σ32-dependent heat shock response in Escherichia coli.
Müller A, Hoffmann JH, Meyer HE, Narberhaus F, Jakob U, Leichert LI. Epub 2013 Apr 12.
J Bacteriol. 2013 Jun;195(12):2807-16. doi: 10.1128/JB.00127-13. PMCID: PMC3697257.(Abstract)

NemR is a Bleach-Sensing Transcription Factor. Gray MJ, Wholey WY, Parker BW, Kim M, Jakob U. March 2013.
J Biol Chem, 288(19):13789-98 (Abstract)

Time line of redox events in aging postmitotic cells. Brandes N, Tienson H, Lindemann A, Vitvitsky V, Reichmann D, Banerjee R, Jakob U. March 2013. elife, 2:e00306 (Abstract)

The roles of conditional disorder in redox proteins. Reichmann D, Jakob U. March 2013.
Curr Opin Struct Biol, pii: S0959-440X(13)00035-3 (Abstract)

Redox Control: A black hole for oxidized glutathione. Winther JR, Jakob U. February 2013.
Nat Chem Biol, 9(2):69-70 (Abstract)

Get3 is a holdase chaperone and moves to deposition sites for aggregated proteins when membrane targeting is blocked. Powis K, Schrul B, Tienson H, Gostimskaya I, Breker M, High S, Schuldiner M, Jakob U, Schwappach B. January 2013. J Cell Sci. 126(Pt 2):473-83 (Abstract)

Conditional disorder in chaperone action. Bardwell JC, Jakob U. December 2012. Trends Biochem Sci, 37(12):517-2 (Abstract)

Quantitative in vivo redox sensors uncover oxidative stress as an early event in life.
Knoefler D, Thamsen M, Koniczek M, Niemuth NJ, Diederich AK, Jakob U. September 2012.
Mol Cell, 47(5):767-7 (Abstract)

Redox, haem and CO in enzymatic catalysis and regulation.
Ragsdale SW, Yi L, Bender G, Gupta N, Kung Y, Yan L, Stich TA, Doukov T, Leichert L, Jenkins PM, Bianchetti CM, George SJ, Cramer SP, Britt RD, Jakob U, Martens JR, Phillips GN Jr, Drennan CL. June 2012.
Biochem Soc Trans, 40(3):501-7 (Abstract)

Hsp33 confers bleach resistance by protecting elongation factor Tu against oxidative degradation in Vibrio cholerae. Wholey WY, Jakob U. March 2012. Mol Microbiol, 83(5):981-91 (Abstract)

Order out of disorder: working cycle of an intrinsically unfolded chaperone.
Reichmann D, Xu Y, Cremers CM, Ilbert M, Mittelman R, Fitzgerald MC, Jakob U. January 2012.
Cell, 148(5):947-57 (Abstract)

Using quantitative redox proteomics to dissect the yeast redoxome.
Brandes N, Reichmann D, Tienson H, Leichert LI, Jakob U. December 2011.
J Biol Chem, 286(48):41893-903 (Abstract)

E. coli chaperones DnaK, Hsp33, and Spy inhibit bacterial functional amyloid assembly.
Evans ML, Schmidt JC, Ilbert M, Doyle SM, Quan S, Bardwell JC, Jakob U, Wickner S, Chapman MR. October 2011.
Prion, 5(4) (Abstract)

Chlorinated phenols control the expression of the multidrug resistance efflux pump MexAB-OprM in Pseudomonas aeruginosa by interacting with NalC. Ghosh S, Cremers CM, Jakob U, Love NG. March 2011.
Mol Microbiology, 79(6):1547-56 (Abstract)

Effects of oxidative stress on behavior, physiology, and the redox thiol proteome ofCaenorhabditis elegans.
Kumsta C, Thamsen M, Jakob U. March 2011. Antiox Redox Signal, 14(6):1023-37 (Abstract)

Genetic selection designed to stabilize proteins uncovers a chaperone called Spy.
Quan S, Koldewey P, Tapley T, Kirsch N, Ruane KM, Pfizenmaier J, Shi R, Hofmann S, Foit L, Ren G, Jakob U, Xu Z, Cygler M, Bardwell JC. March 2011. Nat Struct Mol Biol, 18(3):262-9 (Abstract)

Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation?.
Zhao F, Ilbert M, Varadan R, Cremers CM, Hoyos B, Acin-Perez R, Vinogradov V, Cowburn D, Jakob U, Hammerling U. March 2011. Antioxid Redox Signal, 14(5):757-66 (Abstract)

The redoxome: Proteomic analysis of cellular redox networks. Thamsen M, Jakob U. February 2011. Curr Opin Chem Biol, 15(1):113-9 (Abstract)

Is overoxidation of peroxiredoxin physiologically significant? Thamsen M, Kumsta C, Li F, Jakob U. February 2011. Antioxid Redox Signal, 14(4):725-30 (Abstract)

Unfolding of metastable linker region is at the core of Hsp33 activation as a redox-regulated chaperone.
Cremers CM, Reichmann D, Hausmann J, Ilbert M, Jakob U. April 2010. J Biol Chem, 285(15):11243-51 (Abstract)

Thermodynamic analysis of a molecular chaperone binding to unfolded protein substrates.
Xu Y, Schmitt S, Tang L, Jakob U, Fitzgerald MC. January 2010. Biochemistry, 49(6):1346-53 (Abstract)

Protein refolding by pH-triggered chaperone binding and release. Tapley TL, Franzmann TM, Chakraborty S, Jakob U, Bardwell JC. January 2010. Proc Natl Acad Sci U S A, 107(3):1071-6 (Abstract)

Heme regulatory motifs in heme oxygenase-2 form a thiol/disulfide redox switch that responds to the cellular redox state. Yi L, Jenkins PM, Leichert LI, Jakob U, Martens JR, Ragsdale SW. July 2009.
J Biol Chem. 284(31):20556-61 (Abstract)

Redox-regulated chaperones. Kumsta C, Jakob U. June 2009. Biochemistry, 48(22):4666-76 (Abstract)

Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli.
Barth E, Gora KV, Gebendorfer KM, Settele F, Jakob U, Winter J. May 2009. Microbiology, 155(Pt 5):1680-9 (Abstract)

Structural plasticity of an acid-activated chaperone allows promiscuous substrate binding.
Tapley TL, Körner JL, Barge MT, Hupfeld J, Schauerte JA, Gafni A, Jakob U, Bardwell JC. May 2009.
Proc Natl Acad Sci USA, 106(14):5557-62 (Abstract)

Protein refolding by pH-triggered chaperone binding and release.
Tapley TL, Franzmann TM, Chakraborty S, Jakob U, Bardwell JC. January 2009.
Proc Natl Acad Sci USA, 107(3):1071-6 (Abstract)

Bleach activates a redox-regulated chaperone by oxidative protein unfolding.
Winter J, Ilbert M, Graf PC, Ozcelik D, Jakob U. November 2008. Cell, 135(4):691-701 (Abstract)

Thiol-Based Redox Switches in Eukaryotic Proteins. Brandes N, Schmitt S, Jakob U. November 2008.
Antioxid Redox Signal, [Epub ahead of print]. (Abstract)

Quantifying Changes in the Thiol Redox Proteome Upon Oxidative Stress in Vivo.
Leichert LI, Gehrke F, Gudiseva HV, Ilbert M, Blackwell T, Walker AK, Strahler JR, Andrews PC, Jakob U. June 2008.
Proc Nat Acad Sci, 105(24):8197-202 (Abstract)

Special issue: redox regulation of protein folding. Preface. Herrmann JM, Jakob U. April 2008.
Biochim Biophys Acta, 1783(4):519 (Abstract)

Nitrosative stress treatment of E. coli targets distinct set of thiol-containing proteins.
Brandes N, Rinck A, Leichert LI, Jakob U. November 2007. Mol Microbiol, 66(4):901-14 (Abstract)

The redox-switch domain of Hsp33 functions as dual stress sensor. Ilbert M, Horst J, Ahrens S, Winter J, Graf PC, Lilie H, Jakob U. June 2007. Nat Struct Mol Biol, 14(6):556-63 (Abstract)

XIAP Is a copper binding protein deregulated in Wilson’s disease and other copper toxicosis disorders.
Mufti AR, Burstein E, Csomos RA, Graf PCF, Wilkinson JC, Dick RD, Challa M, Son JK, Bratton SB, Su GL, Brewer GJ, Jakob U, Duckett CS. August 2006. Mol Cell, 21(6):775-85 (Abstract)

Global Methods to Monitor the Thiol-Disulfide State of Proteins in vivo.
Leichert LI, Jakob U. May 2006. Antioxid Redox Signal, 8(5-6):763-72 (Abstract)

CoSMoS: Conserved Sequence Motif Search in the proteome. Liu XI, Korde N, Jakob U, Leichert LIO. January 2006.
BMC Bioinformatics, 7:37 (Abstract)

Severe Oxidative Stress Causes Inactivation of DnaK and Activation of the Redox Regulated Chaperone Hsp33.
Winter J, Linke K, Jatzek A, Jakob U. February 2005. Mol Cell, 17(3):381-92 (Abstract)

Protein Thiol Modifications Visualized in vivo.
Leichert L, Jakob U. November 2004. PloS Biology, 2(11):e333 (Abstract)

Beyond Transcription – Novel Mechanisms to Regulate Molecular Chaperone Activity.
Winter J, Jakob U. September 2004. Critical Review in Biochemistry and Molecular Biology, 39(5-6):297-317 (Abstract)

The Crystal Structure of the Reduced Zn²⁺-Bound Form of the B. subtilis Hsp33 Chaperone and its Implications for the Activation Mechanism. Janda I, Devedjiev Y, Derewenda U, Dauter Z, Bielnicki J, Cooper DR, Graf PCF, Joachimiak A, Jakob U, Derewenda Z. August 2004.Structure, 12(10):1901-7 (Abstract)

The Zinc-dependent Redox Switch Domain of the Chaperone Hsp33 has a Novel Fold. Won HS, Low LY, DeGuzman R, Martinez-Yamout M, Jakob U, Dyson HJ. August 2004. J Mol Biol, 341(4):893-99 (Abstract)

Substrate binding analysis of the 23S rRNA methyltransferase RrmJ. Hager J, Staker B, Jakob U. May 2004. J Bact, 186(19):6634-42 (Abstract)

Activation of the redox regulated chaperone Hsp33 by domain unfolding. Graf PCF, Martinez-Yamout M, VanHaerents S, Lilie H, Dyson JH, Jakob U. April 2004. J Biol Chem, 279(19):20520-38 (Abstract)

Identification of a redox regulated chaperone network. Hoffmann JH, Graf PCF, Linke K, Lilie H, Jakob U.
January 2004. EMBO J, 23(1):160-8 (Abstract)

Redox regulation of chaperones. Hoffmann JH, Jakob U. January 2004.
Protein Folding Handbook. eds. Buchner, J. and Kiefhaber, T., Wiley-VCH, ISBN: 3-527-30784-2.

Thioredoxin 2, an oxidative stress induced protein, contains a high affinity zinc binding site.
Collet JF, D’Souza JC, Jakob U, Bardwell JCA. November 2003. J Biol Chem, 278(46):45325-32 (Abstract)

The roles of the two zinc binding sites in DnaJ. Linke K, Wolfram T, Bussemer J, Jakob U. September 2003.
J Biol Chem, 278(45):44457-66 (Abstract)

Not every disulfide lasts forever: disulfide bond formation as a redox switch.
Linke K, Jakob U. August 2003. Antioxid Redox Signal, 5(4):425-34 (Abstract)

Redox regulated molecular chaperones. Graf PCF, Jakob U. October 2002.
Cell Mol Life Sci, 59(10):1624-31 (Abstract)

Active site in RrmJ, a heat shock induced methyltransferase. Hager J, Staker BL, Bugl H, Jakob U. September 2002.
J Biol Chem, 277:41978-86 (Abstract)

Overexpression of two different GTPases rescues a null mutation in a heat-induced rRNA methyltransferase.
Tan J, Jakob U, Bardwell JCA. January 2002. J Bact, 184(10):2692-8 (Abstract)

The 2.2 Å Crystal Structure of Hsp33: A Heat Shock Protein with Redox-regulated Chaperone Activity.
Vijayalakshm J, Mukherjee M, Graumann J, Jakob U, Saper M. May 2001. Structure, 9(5):367-75 (Abstract)

Activation of the Redox Regulated Molecular Chaperone Hsp33-A Two Step Mechanism.
Graumann J, Lilie H, Tang X, Tucker KC, Hoffmann JH, Vijayalakshmi J, Saper M, Bardwell JCA, Jakob U. January 2001.
Structure, 9(5):377-87 (Abstract)

Hsp33’s Redox Switch has a Novel Zinc-Binding Motif. Jakob U, Eser M, Bardwell JCA. December 2000. J Biol Chem, 275(49):38302-10 (Abstract)

RNA Methylation under Heat Shock Control. Bügl H, Fauman EB, Staker BL, Zheng F, Kusher SR, Saper MA, Bardwell JCA, Jakob U. August 2000. Mol Cell, 6:2)349-60 (Abstract)

Mass Spectrometry Unravels Disulfide Bond Formation as Mechanism to Activate a Molecular Chaperone.
Barbirz S, Jakob U, Glocker MO. June 2000. J Biol Chem, 275(25):18759-66 (Abstract)

DsbG, a Protein Disulfide Isomerase With Chaperone Activity. Shao F, Bader M, Muse W, Jakob U, Bardwell JCA. May 2000. J Biol Chem, 275(18):13349-52 (Abstract)

Heating Greatly Speeds Coomassie Blue Staining and Destaining. Wong C, Bardwell JCA, Jakob U. March 2000.
Biotechniques, 28(3):426-8, 430, 432 (Abstract)

Chaperone Activity with a Redox Switch. Jakob U, Muse W, Eser M, Bardwell JCA. January 1999.
Cell, 96(3):341-52 (Abstract)

Analysis of Chaperone Function Using Citrate Synthase as a Nonnative Substrate. Buchner J, Grallert H, Jakob U. March 1998. Methods Enzymol, 290:323-38 (Abstract)

Purification and Characterization of Pro- and Eukaryotic Hsp90. Buchner J, Bose S, Jakob U. February 1998.
Methods Enzymol, 290:409-18 (Abstract)

Mammalian Hsp90. Jakob U, Buchner J. February 1997.
Guidebook to the Molecular Chaperones and Protein Folding Catalysts. ed. Gething, M.J., Oxford University Press.

HtpG. Jakob U, Bardwell JCA. January 1997.
Guidebook to the Molecular Chaperones and Protein Folding Catalysts. ed. Gething, M.J. Oxford University Press.

Hsp90-News from the Front. Jakob U. October 1996. Frontiers in Bioscience, 1:d309-17 (Abstract)

Assessment of the ATP Binding Properties of Hsp90. Jakob U, Scheibel T, Bose S, Reinstein J, Buchner J . January 1996. J Biol Chem, 271(17):10035-41 (Abstract)

Method for the stabilization of proteins using heat shock protein Hsp90.
Jakob U, Buchner J, Zimmermann R, Rudolph R. March 1995. United States Patent # 5,474,892.

Transient Interaction of Hsp90 with Early Unfolding Intermediates of Citrate Synthase-Implications for Heat Shock in vivo. Jakob U, Lilie H, Meyer I, Buchner J. March 1995. J Biol Chem, 270(13):7288-94 (Abstract)

Structural Organization of Eu- and Procaryotic Hsp90-Influence of Divalent Cations on Structure and Function.
Jakob U, Meyer I, Bügl H, Andrè S, Bardwell JCA, Buchner J. February 1995. J Biol Chem, 270(24):14412-9 (Abstract)

Assisting Spontaneity: The Role of Hsp90 and Small Hsps as Molecular Chaperones.
Jakob U, Buchner J. May 1994. Trends Biochem Sci, 19(5):205-11 (Abstract)

Stress- and Mitogen-Induced Phosphorylation of the Small Heat Shock Protein Hsp25 by MAPKAP Kinase 2 is not Essential for Chaperone Properties and Cellular Thermoresistance. Knauf U, Jakob U, Engel K, Buchner J, Gaestel M. January 1994. EMBO J, 13(1):54-60 (Abstract)

Small Heat Shock Proteins are Molecular Chaperones. Jakob U, Gaestel M, Engel K, Buchner J. January 1993.
J Biol Chem, 268(3):1517-20 (Abstract)

Hsc70, Immunoglobulin Heavy Chain Binding Protein, and Hsp90 Differ in their Ability to Stimulate Transport of Precursor Proteins into Mammalian Microsomes. Wiech H, Buchner J, Zimmermann M, Zimmermann R, Jakob U. January 1993. J Biol Chem, 268(10):7414-21 (Abstract)

Hsp90 Chaperones-Protein Folding in vitro. Wiech H, Buchner J, Zimmermann R, Jakob U. July 1992. Nature, 358(6382):169-70 (Abstract)

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