{"id":463,"date":"2022-08-19T15:01:24","date_gmt":"2022-08-19T19:01:24","guid":{"rendered":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/?page_id=463"},"modified":"2026-03-06T12:52:15","modified_gmt":"2026-03-06T17:52:15","slug":"cryo-em-approaches-for-small-membrane-proteins","status":"publish","type":"page","link":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/cryo-em-approaches-for-small-membrane-proteins\/","title":{"rendered":"Cryo-EM and structural analysis of membrane proteins"},"content":{"rendered":"\n
<\/summary>\n
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\"\"<\/a><\/figure>\n<\/figure>\n<\/details>\n\n\n\n

Ultimately, we want to know how these proteins work on a molecular level. We have ongoing basic research and drug discovery projects that use cryo-EM to gain a mechanistic understanding of protein function. The figures below show two recent structures we have determined, one of a CLC family fluoride exporter, and one from the FEX family of eukaryotic fluoride channels.<\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n
\"\"<\/a><\/figure>\n\n\n\n

Kang C-Y, An M#, Heidari S, Torabifard H, Ohi MD, Stockbridge RB. (2026). The molecular mechanism of fluoride export by the eukaryotic fluoride channel FEX.<\/a> Nat. Comm. <\/em>17:589.<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Ultimately, we want to know how these proteins work on a molecular level. We have ongoing basic research and drug discovery projects that use cryo-EM to gain a mechanistic understanding of protein function. The figures below show two recent structures we have determined, one of a CLC family fluoride exporter, and one from the FEX […]<\/p>\n","protected":false},"author":939,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-463","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/pages\/463","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/users\/939"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/comments?post=463"}],"version-history":[{"count":12,"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/pages\/463\/revisions"}],"predecessor-version":[{"id":802,"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/pages\/463\/revisions\/802"}],"wp:attachment":[{"href":"https:\/\/sites.lsa.umich.edu\/randystockbridge\/wp-json\/wp\/v2\/media?parent=463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}