Publications on Pubmed Google Scholar
2024
Basalla JL, Ghalmi M, Hoang Y, Dow RE, Vecchiarelli AG*. An invariant C-terminal tryptophan in McdB mediates its interaction and positioning function with carboxysomes. BioRxiv
Hoang Y, Azaldegui CA, Dow RE, Ghalmi M, Biteen JS*, Vecchiarelli AG*. An experimental framework to assess biomolecular condensates in bacteria. Nature Communications
Pulianmackal LT, Vecchiarelli AG*. Positioning of cellular components by the ParA/MinD family of ATPases. Current Opinion in Microbiology
2023
Basalla JL, Mak CA, Byrne JA, Ghalmi M, Hoang Y, Vecchiarelli AG*. Dissecting the phase separation and oligomerization activities of the carboxysome positioning protein McdB. Elife
Pulianmackal LT, Limcaoco JMI, Ravi K, Yang S, Zhang J, Tran MK, Ghalmi M, O’Meara MJ, Vecchiarelli AG*. Multiple ParA/MinD ATPases coordinate the positioning of disparate cargos in a bacterial cell. Nature Communications
Swasthi HM, Basalla JL, Dudley CE, Vecchiarelli AG, Chapman MR. Cell surface-localized CsgF condensate is a gatekeeper in bacterial curli subunit secretion. Nature Communications
2021
Beaufay F, Amemiya HM, Guan J, Basalla J, Meinen BA, Chen Z, Mitra R, Bardwell JCA, Biteen JS, Vecchiarelli AG, Freddolino PL, Jakob U. Polyphosphate drives bacterial heterochromatin formation. Science Advances
Landino J, Leda M, Michaud A, Swider ZT, Prom M, Field CM, Bement WM, Vecchiarelli AG, Goryachev AB, Miller AL. Rho and F-actin self-organize within an artificial cell cortex. Current Biology
Hakim P, Hoang Y, Vecchiarelli AG*. Dissection of the ATPase active site of McdA reveals the sequential steps essential for carboxysome distribution. Mol Biol Cell
Rillema R, Hoang Y, MacCready JS, Vecchiarelli AG*. Carboxysome Mispositioning Alters Growth, Morphology, and Rubisco Level of the Cyanobacterium Synechococcus elongatus PCC 7942. mBio
MacCready JS, Vecchiarelli AG*. Positioning the Model Bacterial Organelle, the Carboxysome. mBio
MacCready JS, Tran L, Basalla JL, Hakim P, Vecchiarelli AG*. The McdAB system positions α-carboxysomes in proteobacteria. Mol Microbiol
2020
Groaz A, Moghimianavval H, Tavella F, Giessen TW, Vecchiarelli AG, Yang Q, Liu AP*. Engineering spatiotemporal organization and dynamics in synthetic cells. Wiley Interdiscip Rev Nanomed Nanobiotechnol
Raghunathan S, Chimthanawala A, Krishna S, Vecchiarelli AG, Badrinarayanan A*. Asymmetric chromosome segregation and cell division in DNA damage-induced bacterial filaments. Mol Biol Cell
Azaldegui CA, Vecchiarelli AG*, Biteen JS*. The Emergence of Phase Separation as an Organizing Principle in Bacteria. Biophys J
MacCready JS, Basalla JL, Vecchiarelli AG*. Origin and Evolution of Carboxysome Positioning Systems in Cyanobacteria. Mol Biol Evol
2019
Tarnopol RL, Bowden S, Hinkle K, Balakrishnan K, Nishii A, Kaczmarek CJ, Pawloski T, Vecchiarelli AG*. Lessons from a Minimal Genome: What Are the Essential Organizing Principles of a Cell Built from Scratch? Chembiochem
- All authors are undergraduates from the MCDB 401 class of 2019 “Building the Synthetic Cell”
2018
MacCready JS, Hakim P, Young EJ, Hu L, Liu J, Osteryoung KW, Vecchiarelli AG*, Ducat DC*. Protein gradients on the nucleoid position the carbon-fixing organelles of cyanobacteria. eLife
* Co-corresponding authors
MacCready JS & Vecchiarelli AG*. In long bacterial cells, the Min system can act off-center. Mol Micro
Mizuuchi K & Vecchiarelli AG*. Mechanistic insights of the Min oscillator via cell-free reconstitution and imaging. Phys Biol
2017
Hu L, Vecchiarelli AG, Mizuuchi K, Neuman KC & Liu J. Brownian ratchet mechanisms of ParA-mediated partitioning. Plasmid
Hu L, Vecchiarelli AG, Mizuuchi K, Neuman KC & Liu J. Brownian Ratchet Mechanism for Faithful Segregation of Low-Copy-Number Plasmids. Biophys J
Pre-University of Michigan
2016
Vecchiarelli AG, Li M, Mizuuchi M, Hwang LC, Seol Y, Neuman KC & Mizuuchi K.Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinD. PNAS
- Commentary highlight – Sherratt D. Oscillation helps to get division right. PNAS
2015
Longhua Hu, Vecchiarelli AG, Mizuuchi K, Neuman KC & Liu J. Directed and persistent movement arises from mechanochemistry of the ParA/ParB system. PNAS, E7055
Vecchiarelli AG*, Taylor JA & Mizuuchi K. ParAB motility reconstitution. Building a Cell from its Component Parts. Methods in Cell Biology 128, Chapter 13
* Corresponding author
Vecchiarelli AG, Seol Y, Neuman KC & Mizuuchi K. A moving ParA gradient on the nucleoid directs subcellular cargo transport via a chemophoresis force. BioArchitecture 4, 154
2014
Vecchiarelli AG, Li M, Mizuuchi M & Mizuuchi K. Differential affinities of MinD and MinE to anionic phospholipid influence Min patterning dynamics in vitro. Mol Microbiol 93, 453
Vecchiarelli AG, Neuman KC & Mizuuchi K. A propagating ATPase gradient drive transport of surface-confined cellular cargo. PNAS 111, 4880
- Commentary highlight – Kiekebusch D and Thanbichler M. Plasmid segregation by a moving ATPase gradient. PNAS 111, 4741
- Winner of the 2014 Cozzarelli Prize at PNAS, division of Biological Sciences
- PNAS Science Sessions Podcast
2013
Vecchiarelli AG, Havey JC, Ing L, Wong E, Waples W & Funnell BE. Dissection of the ATPase active site of P1 ParA reveals multiple active forms essential for plasmid partition. J Biol Chem 288, 17823
Vecchiarelli AG, Hwang LC & Mizuuchi K. Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism. PNAS 110, E1390
Hwang LC*, Vecchiarelli AG*, Han YW, Mizuuchi M, Harada Y, Funnell BE & Mizuuchi K. ParA-mediated plasmid partition driven by protein pattern self-organization.EMBO J 32, 1238
* Equal Contribution
- Commentary highlight – Sherratt D. Plasmid partition: sisters drifting apart.EMBO 32,1208
Vecchiarelli AG & Funnell BE. Probing the N-terminus of ParB using cysteine-scanning mutagenesis & thiol modification. Plasmid 70, 86
2012
Vecchiarelli AG, Mizuuchi K & Funnell BE. Surfing biological surfaces: exploiting the nucleoid for partition and transport in bacteria. Mol Microbiol 86, 513
- Rated a “Must Read” by the Faculty of 1000
Havey JC, Vecchiarelli AG & Funnel BE. ATP-regulated interactions between P1 ParA, ParB & non-specific DNA that are stabilized by the plasmid partition site. Nucl Acids Res 40, 801
2010
Vecchiarelli AG, Han YW, Tan X, Mizuuchi M, Ghirlando R, Biertümpfel C, Funnell BE & Mizuuchi K. ATP control of dynamic P1 ParA-DNA interactions: a key role for the nucleoid in plasmid partition. Mol Microbiol 78, 78
- Commentary highlight – Howard M & Gerdes K. What is the mechanism of ParA-mediated DNA movement? Mol Microbiol 78, 9
- 2010 highlight from the ASM blog “Small Things Considered”
2007
Vecchiarelli AG, Schumacher MA & Funnell BE. P1 partition complex assembly involves several modes of protein-DNA recognition. J Biol Chem 282, 10944
