Western Lake Erie Culture Collection

Overview

The Western Lake Erie Culture Collection (WLECC) contains 21+ xenic Microcystis cultures isolated from the western basin of Lake Erie from 2017-present. Researchers can submit requests for culture isolates from the WLCC by sending an email to GreatLakesCC@umich.edu or using the form on the Available Cultures page below.

Current Team

Sara R. Rivera

Lab Manager

sarariv@umich.edu

Nicole Rappuhn

Lab Tech

nrappuhn@umich.edu

Lindsey Gleason

Assistant Researcher

nitschky@umich.edu

Data

Raw reads, and metagenome assembled genomes (MAGs) were deposited at the respective NCBI repositories and are available in BioProject PRJNA903891. Raw reads are available under the SRA accession numbers: SRR22360640-SRR22360660.

Publications

  • Yancey, C. E., Kiledal, E. A., Chaganti, S. R., Denef, V. J., Errera, R. M., Evans, J. T., … & Dick, G. J. (2023). The Western Lake Erie Culture Collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome. Harmful Algae, 102440. https://doi.org/10.1016/j.hal.2023.102440
  • Baker, D., Godwin, C. M., Khanam, M., Burtner, A. M., Dick, G. J., & Denef, V. J. (2023). Variation in resource competition traits among Microcystis strains is affected by their microbiomes. Mlife2(4), 401-415. https://doi.org/10.1002/mlf2.12094
  • Yancey, C. E., Hart, L., Hefferan, S., Mohamed, O. G., Newmister, S. A., Tripathi, A., … & Dick, G. J. (2024). Metabologenomics reveals strain-level genetic and chemical diversity of Microcystis secondary metabolism. Msystems, e00334-24. https://doi.org/10.1128/msystems.00334-24
  • Kiledal, E. A., Reitz, L. A., Kuiper, E. Q., Evans, J., Siddiqui, R., Denef, V. J., & Dick, G. J. (2024). Comparative genomic analysis of Microcystis strain diversity using conserved marker genes. Harmful Algae132, 102580. https://doi.org/10.1016/j.hal.2024.102580
  • Li, W., Baliu-Rodriguez, D., Premathilaka, S. H., Thenuwara, S. I., Kimbrel, J. A., Samo, T. J., … & Mayali, X. (2024). Microbiome processing of organic nitrogen input supports growth and cyanotoxin production of Microcystis aeruginosa cultures. The ISME Journal18(1), wrae082. https://doi.org/10.1093/ismejo/wrae082

Funding

  1. Cooperative Institute of Great Lake Research (NA17OAR4320152)
  2. Michigan Sea Grant College Program, project number R/WQ-8, under NA18OAR4170102, from NOAA National Sea Grant, U.S. Department of Commerce and funds from the State of Michigan
  3. National Oceanic and Atmospheric Administration’s National Centers for Coastal Ocean Science under award NA17NOS4780186 via a subcontract from the Ohio State University to the University of Michigan
  4. Air Force Office of Scientific Research grant (DURIP 14RT0605)
  5. Lawrence Livermore National Laboratory LDRD project 20-ERD-061. Part of this work was carried out at LLNL under Contract DE-AC52-07NA27344.
  6. National Science Foundation grant OCE 1736629