The Horowitz Group is working toward uncovering the fundamental limitations that constrain the interplay between energy, thermodynamics, and information in living organisms. The ultimate goal is to develop a predictive nonequilibrium theoretical framework that can inform us about the thermodynamic design principles underlying the structure, function, and operation of life. To accomplish this goal, we draw on theoretical tools from statistical mechanics, stochastic thermodynamics, and large deviation theory, combined with numerical modeling.