Single-Molecule Fluorescence Imaging for Real-Time Super-Resolution Measurements in Microbiology and Nanophotonics

Molecules and their chemical interactions lie at the heart of the world around us. To understand biology, physics, and materials science on the molecular scale, it is therefore imperative that we bridge the gap between the angstrom-to-nanometer scale of molecules and the micron scale of traditional optical microscopy. Our research group is addressing this fundamental challenge by developing single-molecule fluorescence imaging as a flexible, general technology for ultrahigh-resolution, real-time imaging.

Single-molecule methods have had high impact across the disciplines because they enable high-sensitivity measurements, eliminate heterogeneity in characterization, resolve positions on the nanometer scale, and characterize motions and features in situ—even in live cells. With these approaches, we are answering fundamental questions in bacterial cell biology and metal nanoparticle plasmonics.

Biteen Lab Summer 2014 FIT23148_crop_smaller Picture7 Picture8 Picture6 Picture5 Picture3 Picture4 Picture2 Picture1 Picture9
Biteen Lab Summer 2014
The optics table
Single-molecule plasmon-enhanced fluorescence in living bacteria cells
Resolving fast, confined diffusion in bacteria
Plasmon-enhanced emission from intrinsically fluorescent proteins
Identifying mislocalized emission from single molecules near gold nanoparticles
A mechanism for membrane-bound transcription activation in the V. cholerae virulence pathway
Modeling starch-induced assembly by outer-membrane proteins in human gut bacteria
Understanding in vivo target search dynamics in DNA mismatch repair
Single-molecule fluorescence imaging beats the diffraction limit of light
Accessing confined spaces in nanodevices and nanomaterials