Research

To accomplish our goal of understanding the world around us one molecule at a time, the Biteen Lab pursues three closely integrated research thrusts:

(1) Answering fundamental questions about microbial cell biology with single-molecule and super-resolution imaging of biomolecular motions in living bacteria.

Recent Representative Publications

  • H.E. Chia, T. Zuo, N.M. Koropatkin, E.N.G. Marsh and J.S. Biteen, “Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins,” Current Research in Microbial Sciences, 1 1-6 (2020). DOI
  • N. Al-Husini, et al., “BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates,” Molecular Cell, 78 670-682 (2020). DOI
  • H.H. Tuson, M.H. Foley, N.M. Koropatkin* and J.S. Biteen*, “The Starch Utilization System Assembles around Stationary Starch-Binding Proteins,” Biophysical Journal 114 242-250 (2018). DOI
  • Y. Liao, Y. Li, J.W. Schroeder, L.A. Simmons* and J.S. Biteen*, “Single-molecule DNA polymerase dynamics at a bacterial replisome in live cells,” Biophysical Journal, 111 2562-2569 (2016). DOI

(2) Understanding the light-matter interactions that govern plasmon-coupled fluorescence with single-molecule and single-particle investigations of dye/nanoparticle systems.

Recent Representative Publications

  • S.A. Lee and J.S. Biteen, “Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles,” Journal of Physical Chemistry Letters, 10 5764-5769 (2019). DOI
  • T. Zuo, H.J. Goldwyn, B.P. Isaacoff, D.J. Masiello* and J.S. Biteen*, “Rotation of Single-Molecule Emission Polarization by Plasmonic Nanorods,” Journal of Physical Chemistry Letters, 10 15047-5054 (2019). DOI.
  • S.A. Lee and J.S. Biteen, “Interplay of Nanoparticle Resonance Frequency and Array Surface Coverage in Live-Cell Plasmon-Enhanced Single-Molecule Imaging,” Journal of Physical Chemistry C 122 5705-5709 (2018). DOI
  • B. Fu, B.P. Isaacoff, and J.S. Biteen, “Super-Resolving the Actual Position of Single Fluorescent Molecules Coupled to a Plasmonic Nanoantenna,” ACS Nano 11 8978-8987 (2017). DOI

(3) Pioneering new methods to extend the scope of single-molecule imaging to fast, directional, and confined movements in microbiology and nanotechnology.

Recent Representative Publications

  • J.D. Karslake, E.D. Donarski, S.A. Shelby, L.M. Demey, V.J. DiRita, S.L. Veatch and J.S. Biteen, “SMAUG: Analyzing single-molecule tracks with nonparametric Bayesian statistics,” Methods, in press (2020). DOI; Accompanying code on Github
  • B.P. Isaacoff, Y. Li, S.A. Lee and J.S. Biteen, “SMALL-LABS: An algorithm for measuring single-molecule intensity and position in the presence of obscuring backgrounds,” Biophysical Journal, 116 975-982 (2019). DOI. Accompanying code on Github.
  • D.J. Rowland and J.S. Biteen, “Measuring molecular motions inside single cells with improved analysis of single-particle trajectories,” Chemical Physics Letters, 674 173-178 (2017). DOI

Our experiments rely on deep understanding, careful sample preparation, quantitative analysis, and of course, lasers:

laser-epic-crop

Biteen Lab Summer 2011