Publications

Kolli D, Rout SK, Riek R, Chapman MR (2025) The Evolution of Functional Amyloids and Their Impact on Host-Microbe Interactions. Adv Sciences. Pubmed

Balistreri A*, Kolli D*, Jayaweera SJ, Lundahl D, Han Y, Kalcec L, Goetzler E, Alessio R, Ruotolo B, Olofsson A, Chapman MR (2025) The bacterial chaperone CsgC inhibits functional amyloid CsgA formation by promoting the intrinsically disordered pre-nuclear state. Journal of Biological Chemistry. Pubmed

Jessel K, Chapman MR (2024) Microbial warfare: B. subtillis antagonizes E. coli biofilm formation. Trends in micro. Pubmed

Zanditenas E, Trebicz-Geffen M, Kolli D, Domínguez-García L, Farhi E, Linde L, Romero D, Chapman MR, Kolodkin-Gal I, Ankri S. (2023) Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity. NPJ Biofilms Microbiomes. Pubmed

Swasthi H, Basalla J, Dudley, C, Vecchiarelli A, Chapman MR (2023)  Cell surface-localized CsgF condensate is a gatekeeper in bacterial curli subunit secretion. Nat Commun. Pubmed

Bhoite S, Kolli D, Gomulinski MA, Chapman MR (2023) Electrostatic interactions mediate the nucleation and growth of a bacterial functional amyloid. Front Mol Biosci. PubMed

Bhoite S, Han Y, Ruotolo B, and Chapman MR (2022) Mechanistic insights into accelerated α-synuclein aggregation mediated by human microbiome-associated functional amyloids. J Biol Chem. PubMed

Das T, Blasco-Conesa M, Korf J, Honarpisheh P, Chapman MR, Ganesh B (2022) Bacterial Amyloid Curli Associated Gut Epithelial Neuroendocrine Activation Predominantly Observed in Alzheimer’s Disease Mice with Central Amyloid-β Pathology. J Alzheimers Dis. PubMed

Balistreri A, Goetzler E, Chapman MR (2020) Functional Amyloids Are the Rule Rather Than the Exception in Cellular Biology. Microorganisms. PubMed

Balistreri A, Kahana E, Janakiraman S, Chapman MR (2020) Tuning Functional Amyloid Formation Through Disulfide Engineering. Front Microbiol. PubMed

Sampson TR, Challis C, Jain N, Moiseyenko A, Ladinsky MS, Shastri GG, Thron T, Needham BD, Horvath I, Debelius JW, Janssen S, Knight R, Wittung-Stafshede P, Gradinaru V, Chapman MR, Mazmanian SK (2020) A gut bacterial amyloid promotes a-synuclein aggregation and motor impairment in mice. eLife  PubMed

Bhoite S, van Gerven N, Chapman MR, and Remaut H (2019) Curli Biogenesis: Bacterial Amyloid Assembly by the Type VIII Secretion Pathway. EcoSal Plus PubMed

Andreasen M, Meisl G, Taylor JD, Michaels TCT, Levin A, Otzen DE, Chapman MR, Dobson CM, Matthews SJ, and Knowles TPJ (2019) Physical Determinants of Amyloid Assembly in Biofilm Formation. mBio PubMed

Nhu NTK, Phan MD, Peters KM, Lo AW, Forde BM, Min Chong T, Yin WF, Chan KG, Chromek M, Brauner A, Chapman MR, Beatson SA, Schembri MA (2018) Discovery of New Genes Involved in Curli Production by a Uropathogenic Escherichia coli Strain from the Highly Virulent O45:K1:H7 Lineage. mBio PubMed

Klein, RD, Shu Q, Cusumano ZT, Nagamatsu K, Gualberto NC, Lynch AJL, Wu C, Wang W, Jain N, Pinkner, JS, Amarasinghe GK, Hultgren SJ, Frieden C, Chapman MR (2018) Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation. mBio PubMed

Evans ML, Gichana E, Zhou Y, and Chapman MR (2018) Bacterial Amyloids. Methods in molecular biology (Clifton, NJ) PubMed

Deshmukh M, Evans ML, and Chapman MR (2018) Amyloid by Design: Intrinsic Regulation of Microbial Amyloid Assembly. Journal of molecular biology PubMed

Price JE, Chapman MR (2018) Phaged and Confused by the Biofilm Matrix. Nat Microbiol 3:2-3 PubMed

Hufnagel DA, Price  JE, Stephenson RE, Kelley J, Benoit MF, Chapman MR (2018) Thiol starvation induces redox-mediated dysregulation of E. coli biofilm components.  J Bacteriol PubMed

Friedland RP, and Chapman MR (2017) The role of microbial amyloid in neurodegeneration. PLoS pathogens PubMed

Jain N., Ådén J, Nagamatsu K, Evans ML Li, X, McMichael B., Ivanova MI., Almqvist F., Buxbaum J. N., Chapman MR  (2017) Inhibition of curli assembly and E. coli biofilm formation by the human systemic amyloid precursor transthyretin. Proc Natl Acad Sci U S A PubMed

Smith DR, Price JE, Burby PE, Blanco LP, Chamberlain J, Chapman MR (2017) The production of curli amyloid fibers is deeply integrated into the biology of E. coli. Biomolecules 7 PubMed

Hufnagel DA, Evans ML, Greene SE, Pinkner JS, Hultgren SJ, Chapman MR (2016) CRP-cAMP regulates csgD and biofilm formation by uropathogenic Escherichia coli. J Bacteriol 198:3329-3334. PubMed

Horowitz S, Koepnick B, Martin R, Tymieniecki A, Winburn AA, Cooper S, Flatten J, Rogawski DS, Koropatkin NM, Hailu TT, Jain N, Koldewey P, Ahlstrom LS, Chapman MR et al. and Bardwell JC (2016). Determining crystal structures through crowdsourcing and coursework. Nat Commun. 7, 12549. PubMed

Cremers CM, Knoefler D, Gates S, Martin N, Dahl JU, Lempart J, Xie L, Chapman MR, Galvan V, Southworth DR et al. and Jakob U (2016). Polyphosphate: A Conserved Modifier of Amyloidogenic Processes. Mol Cell. 63, 768-780. PubMed

Floyd KA, Mitchell C, Eberly AR, Colling S, Zhang EW, DePas W, Chapman MR, Conover M, Rogers BR, Hultgren SJ and Hadjifrangiskou M (2016). The UbiI (VisC) aerobic ubiquinone synthase is required for expression of type 1 pili, biofilm formation, and pathogenesis in uropathogenic Escherichia coli. J. Bacteriology. 198(19): 2262-72. PubMed 

Spaulding CN, Dodson KW, Chapman MR, Hultgren SJ (2015) Fueling the Fire with Fibers: Bacterial Amyloids Promote Inflammatory Disorders. Cell Host Microbe, 18: 1–2. PubMed

Taylor JD, Hawthorne WJ, Lo J, Dear A, Meisl G, Andreasen M, Fletcher C, Koch M, Darvill N, Scull N, Escalera-Maurer A, Sefer L, Wenman R, Lambert S, Jean J, Xu Y, Turner B, Kazarian SG, Chapman MR, Bubeck D, de Simone A, Knowles TP, Matthews SJ. 2016. Electrostatically-guided inhibition of Curli amyloid nucleation by the CsgC-like family of chaperones.  Sci Rep 6:24656. dos: 10.1038/srep24656. PubMed

Chorell E, Andersson E, Evans ML, Jain N, Götheson A, Åden J, Chapman MR, Almqvist F, Wittung-Stafshede P. 2015. Bacterial Chaperones CsgE and CsgC Differentially Modulate Human alpha-Synuclein Amyloid Formation via Transient Contacts. PLoS One 10(10):e0140194. dos:10.1371/journal.pone.0140194. PubMed

Hufnagel DA, DePas WH, Chapman MR. 2015. The biology of the Escherichia coli extracellular matrix. Microbiol Spectrum 3(3):MB-0014-2014. doi:10.1128/microbiolspec.MB -0014-2014. PubMed

Evans ML, Chorell E, Taylor JD, Åden J, Götheson A, Li F, Koch M, Sefer L, Matthews SJ, Wittung-Stafshede P, Almqvist F, Chapman MR. (2015). The bacterial curli system possesses a potent and selective inhibitor of amyloid formation. Mol Cell. 57(3):445-55. PubMed

DePas WH, Syed AK, Sifuentes M, Lee JS, Warshaw D, Saggar V, Csankovszki G, Boles BR, and Chapman MR. (2014). Biofilm Formation Protects Escherichia coli against Killing by Caenorhabditis elegans and Myxococcus xanthus. Appl Environ Microbiol. PubMed

Goyal P, Krasteva PV, Van Gerven N, Gubellini F, Van den Broeck I, Troupiotis-Tsailaki A, Jonckheere W, Pehau-Arnaudet G, Pinkner JS, Chapman MR, et al. (2014). Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG. Nature. *corresponding author is Han Remaut PMID: 25219853 PubMed

Andersson, EK., Bengtsson, C., Evans, ML., Chorell, E., Sellstedt, M., Lindgren, A.E., Hufnagel, DA, Bhattacharya, M., Tessier, P.M., Wittung-Stafshede, P., Almqvist, F., Chapman, MR. (2013). Modulation of Curli Assembly and Pellicle Biofilm Formation by Chemical and Protein Chaperones. Chem Biol. 20(10):1245-54. PubMed

Evans, ML., Chapman, MR. (2013). Curli Biogenesis: Order out of Disorder. Biochim Biophys Acta.10.1016/j.bbamcr.2013.09.010. PubMed

Hufnagel, DA., Tükel, Ç., Chapman, MR. (2013). Disease to Dirt: The Biology of Microbial Amyloids. PLoS Pathog. 9(11):e1003740. PubMed

Hung, C., Zhou, Y., Pinkner, JS., Dodson, KW., Crowley, JR., Heuser, J., Chapman, MR., Hadjifrangiskou, M., Henderson, JP., Hultgren, SJ. (2013). Escherichia coli Biofilms Have an Organized and Complex Extracellular Matrix Structure. MBio. 4(5):e00645-13. PubMed

DePas, WH Hufnagel, DA Lee, JS Blanco, LP Bernstein, HC Fisher, ST James, GA Stewart, PS Chapman, MR. (2013).  Iron induces bimodal population development by Escherichia coli. Proc Natl Acad Sci U S A.110(7)2629-34. PubMed

Zhou, Y Smith, DR Hufnagel, DA Chapman, MR. (2013).  Experimental manipulation of the microbial functional amyloid called curli. Methods Mol Biol. 966 53-75. PubMed

Andersson, EK Chapman, MR. (2013).  Small molecule disruption of B. subtilis biofilms by targeting the amyloid matrix. Chem Biol. 20(1)5-7. PubMed

Hartman, K Brender, JR Monde, K Ono, A Evans, ML Popovych, N Chapman, MR Ramamoorthy, A. (2013).  Bacterial curli protein promotes the conversion of PAP248-286 into the amyloid SEVI: cross-seeding of dissimilar amyloid sequences. Peer J. 1 (e5). PubMed

Zhou, Y Smith, D Leong, BJ Brannstrom, K Almqvist, F Chapman, MR. (2012).  Promiscuous cross-seeding between bacterial amyloids promotes interspecies biofilms. J Biol Chem. 287(47):35092-103. PubMed

Neal D. Hammer, Bryan A. McGuffie, Yizhou Zhou, Matthew P. Badtke, Ashley A. Reinke, Kristoffer Brännström, Jason E. Gestwicki, Anders Olofsson, Fredrik Almqvist, Matthew R. Chapman. (2012). The C-terminal repeating units of CsgB direct bacterial functional amyloid nucleation. Journal of Molecular Biology. 422(3):376-89. PubMed

Blanco L, Evans M, Smith D, Badtke M, Chapman MR. (2012). Diversity, biogenesis and function of microbial amyloids. Trends Microbiol. 20, 66-73. PubMed

Zhou, Y Blanco, LP Smith, DR Chapman, MR. (2012).  Bacterial amyloids. Methods Mol Biol. 849:303-320. PubMed

Horvath I, Weise C, Andersson E, Chorell E, Sellstedt M, Bengtsson C, Olofsson A, Hultgren S, Chapman MR, Wolf-Watz M, Almqvist F, Wittung Stafshede P. (2012). Mechanisms of Protein Oligomerization: Inhibitor of Functional Amyloids Templates alpha-Synuclein Fibrillation. JACS. 134(7):3439-44. PubMed

Evans M, Schmidt J, Ilbert M, Doyle S, Quan S, Bardwell J, Jakob U, Wickner S, Chapman MR. (2011). E. coli chaperones DnaK, Hsp33 and Spy inhibit bacterial functional amyloid assembly. Prion. 5(4):323-34.

Taylor J, Zhou Y, Salgado P, Patwardhan A, Mcguffie M, Pape T, Grabe G, Ashman E, Constable S, Simpson P, Lee WC, Cota E, Chapman MR*, Matthews S*. (2011). Atomic resolution insights into curli fiber biogenesis. Structure . 19, 1307-1316. *co-corresponding authors.

Dueholm M, Nielsen S, Hein K, Nissen P, Chapman MR, Christiansen G, Nielsen P, Otzen D. (2011). Fibrillation of the Major Curli Subunit CsgA under a Wide Range of Conditions Implies a Robust Design of Aggregation. Biochemistry. 50(39):8281-90 PubMed

Nenninger A, Robinson L, Hammer N, Epstein E, Badtke M, Hultgren S, Chapman MR. (2011). CsgE is a curli secretion specificity factor that prevents amyloid fibre aggregation. Mol Microbiol. 81(2):486-99 . PubMed

Kai-Larsen Y, Luthje P, Chromek M, Peters V, Wang X, Holm A, Kadas L, Hedlund K, Johansson J, Chapman M, Jacobson S, Romling U, Agerberth B, Brauner A. . (2010). Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37. PLoS Pathog. 6(7):e1001010 PubMed

Wang X Zhou Y Ren JJ Hammer ND Chapman MR. (2010).  Gatekeeper residues in the major curlin subunit modulate bacterial amyloid fiber biogenesis. PNAS. 107, 163-168.

Cegelski L Pinkner JS Hammer ND Cusumano C Hung CS Chorell E Aberg V Walker JN Seed PC Almqvist F Chapman MR Hultgren SJ. (2009).  Small-molecule inhibitors target Escherichia coli amyloid biogenesis and biofilm formation. Nat Chem Biol. 5:913-919. PubMed

Badtke, MP Hammer, ND Chapman, MR. (2009).  Functional amyloids signal their arrival. SCIENCE SIGNAL. 2(80): 43. PubMed

Ashman Epstein, E, Reizian, MA and Chapman, MR. (2009). Spatial clustering of the curlin secretion lipoprotein requires curli fiber assembly. J of Bacteriol. 191(2): 608-615. PubMed

Wang, X and Chapman, MR. (2008). Curli Provide the Template for Understanding Controlled Amyloid Propagation. Prion. 2(2): 57-60. PubMed

Wang, X and Chapman, MR. (2008). Sequence Determinants of Bacterial amyloid Formation. Journal of Molecular Biology. 380(3): 570-580. PubMed

Wang, X Hammer, ND Chapman, MR. (2008). The molecular basis of functional amyloid polymerization and nucleation. Journal of Biological Chemistry. 283(31): 21530-21539. PubMed

Ashman Epstein E and Chapman MR. (2008). Polymerizing the Fiber Between Host Cells and Bacteria: The Biogenesis of Functional Amyloids. Cellular Microbiology. 10(7):1413-20. PubMed

Hammer ND, Wang X, McGuffie BA, and Chapman MR. (2008). Amyloids: Friend or Foe?. Journal of Alzheimers Disease. 13(4): 407-419. PubMed

Thanassi DG, Chapman MR and Chakraborty S. (2008). Assembly and Secretion of Surface Fibers in Gram-negative Bacteria. In. Bacterial Secreted Proteins. Wooldridge K, ed. Horizon Scientific Press, UK.

Hammer ND, Schmidt JC, Chapman MR. (2007). The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization. PNAS. 104:12494-12499, PubMed

Wang X, Smith D, Jones J, Chapman MR. (2007). In vitro polymerization of a functional E.coli amyloid protein. Journal of Biological Chemistry. 282(6);3713-3719. PubMed

Barnhart M, Chapman MR. (2006). Curli Biogenesis and Function . Annu Rev Microbiol. 60, 131-147. PubMed

Barnhart M, Lynem J, Chapman MR. (2006). GlcNAc-6P Levels Modulate the Expression of Curli Fibers by Escherichia coli. J Bacteriol. 188, 5212-5219. PubMed

Robinson L.S., Ashman Epstein EM, Hultgren S.J., Chapman M.R.. (2006). Secretion of curli fibre subunits is mediated b the outer membrane-localized CsgG protein. Mol Micro. 59(3): 870-881. PubMed

Curli-related Papers Prior to Coming to Michigan

Chapman M.R., Robinson L.S., Pinkner J.S., Roth R., Heuser J., Hammar M., Normark S., Hultgren S.J. (2002). Role of E. coli Curli Operons in Directing Amyloid Fiber Formation. Science. 295: 851-855.

Chapman M.R., Robinson L.S., Hultgren S.J. (2002). The E. coli How-to Guide for Amyloid Formation. ASM News. 69: 121-126.

Chapman M.R., Robinson L.S., Pinkner J.S., Roth R., Heuser J., Hammar M., Normark S., Hultgren S.J. (2002). Role of E. coli Curli Operons in Directing Amyloid Fiber Formation. Science. 295: 851-855.

Justice S, Dodsogn K, Chapman MR, Barnhart MM, Hultgren SJ. (2002). Assembly of Adhesive Organelles on Gram Negative Bacteria. Oudega B (ed.), Protein Secretion Pathways in Bacteria. Boston: Kluwer Academic Publishers.

Roberts JA, Kaack MB, Baskin G, Chapman MR, Hunstad DA, Pinkner JS, Hultgren SJ. (2004). Antibody Responses and Protection from Pyelonephritis Following Vaccination with Purified Escherichia coli PapDG protein. J. Urology. 171:1682-1685.