We use a wide array of techniques and methodologies to accomplish our research goals. Some of them are listed below:
We use several molecular biology techniques including restriction-based molecular cloning, PCR, site-directed mutagenesis, DNA/RNA isolation from human cells, cDNA synthesis, real time PCR etc…
Protein expression and purification
We express proteins/complexes recombinantly in various systems including E. coli, baculovirus-infected insect cells, and cultured human cells for use in biochemical and X-ray crystallographic experiments.
We employ a wide-range of biochemical assays for determining binding constants, enzymatic activity etc. of various telomeric and telomerase complexes. Some examples are shown below.
We use X-ray crystallography as a major tool for determining the molecular bases of the various biological and biochemical phenomena that we investigate.
We use a wide range of cell biological assays ranging from immunofluorescence (IF) and fluorescence in situ hybridization (FISH) imaging of fixed human cells in culture, telomere length analysis, immunoprecipitation (IP), ChIP etc… We also use state-of-the-art techniques to engineer cell lines expressing genetic constructs of our interests in a stable and controlled fashion. Most recently we have successfully used CRISPR-Cas9 technology to introduce both knock out and knock in (single-site changes) mutations in telomeric genes in cultured human cells. Some examples of methods used in the lab are shown below.