Ruotolo Group at Karle Symposium 2018 & Congratulations to Daniel Polasky and Sugyan Dixit

The Ruotolo group presented several posters at the Chemistry departments annual Karle Symposium at the University of Michigan.

We congratulate Daniel Polasky and Sugyan Dixit for winning two of the five travelling awards given to graduate students in the analytical cluster.

We also wish to thank the Karle Symposium Committee, and the two members from our lab Kristine Parson and Daniel Vallejo, for their work on putting together this years symposium.

We look forward to updating our fellow graduate students on our exciting research at next years symposium!

KP_Karle18' DV_Karle18' SD_Karle18'
Kristine Parson presenting her work on nanodiscs
Daniel Vallejo presenting his research on CIU of antibodies
Sugyan Dixit discussing his research with another graduate student in the Chemistry Department

Ruotolo Group at ASMS 2018

The current Ruotolo group enjoying a reunion with alumni at ASMS 2018. (Front to back) Left row: James, Suggie, Brandon, Linjie, Molly, Daniel, Varun; Right row: Chunyi, Yuwei, Jess, Sarah, Carolina, Dan, Michael.

The Ruotolo Group at the ASMS 2018 closing ceremony on the USS Midway. (left to right) back row : Varun, Sarah, Suggie, Dan; Front Row: Chunyi, Daniel, Yuwei, Carolina

Below are the exciting research topics presented at ASMS from our group.

Yuwei Tian – ThOD: Novel Hydrogen/Deuterium Exchange Mass Spectrometry Method for Biopharmaceutical Characterization at High Concentrations

Daniel A. Polasky – TOB: CIUSuite 2: Next-Generation Software for the Analysis of Gas-Phase Protein Unfolding Data

Sugyan Dixit – ThOH: Ion Mobility-Mass Spectrometry and Hybrid Computational Modeling Reveal Detailed Models of Amyloid Peptide Membrane Interactions

Varun V. Gadkari – TP 428: Ion Mobility-Mass Spectrometry Based Characterization of MOAG-4: An Intrinsically Disordered Protein Implicated in Neurodegenerative Disease

Sarah M. Fantin – WP 434: Classifying Ligand Binding Behavior of the Integral Membrane Translocator Protein (TSPO) via Collision Induced Unfolding from Micelles and Nanodiscs

Chunyi Zhao – TP 425: Gas-Phase Unfolding Reveals Stability Shifts in Substrate-Bound Modular Polyketide Synthases

Daniel D. Vallejo – TP 417: Rapid, Parallelized Collision Induced Unfolding of Intact Antibodies: The Influence of Excipients on Antibody Charge Stripping

Carolina Rojas Ramirez – TP 549: Negative-Ion Mode Mass Spectrometry for Revealing Interaction Sites within RNA-Protein Complexes by Selective Infrared Multiphoton Dissociation of RNAPeptide Crosslinked Species

Michael Keating –  TP 757 Altering the Charge-Pairing Environment within Tagged Protein Complexes for Improved Top-Down Sequencing

Brandon promoted to Professor of Chemistry

It’s official! The Umich Regents approved the promotion for Brandon to Professor of Chemistry. Congratulations to Brandon for this wonderful milestone as it recognizes his considerable research contributions to the field of mass spectrometry and structural biology, his service as both a mentor and teacher, and his many contributions within the College of Literature, Science, and the Arts and beyond.

Coarse grain modeling: On computer and wood

IM-MS and integrative modeling reveals the structural model for 18 subunit Urease activation complex

Eschweiler et al recently published the work on deriving the structural model of the Urease activation complex, comprised of 18 subunits, using data from ion mobility mass spectrometry and integrative modeling.

One of the lab members and co-author in the paper, Sugyan, loved the coarse representation of such huge protein complex that he decided to display the model in art form. Here is the wood burnt coarse grain model of the most abundant model from the integrative modeling studies.

Urease activation complex drawn on wood using wood burning technique by Suggie (Sugyan Dixit)

1st Advancing Mass Spectrometry for Biophysics and Structural Biology Conference Launches at UM

AMS Conference photograph, 116 scientists from 9 different countries were represented at the kick-off meeting at UM July 28th- Aug 1st.

From July 28th to August 1st,  The University of Michigan Department of Chemistry played host to a new scientific conference that seeks to bridge the growing needs of the biopharmaceutical industry with the an emerging area of academic research focused on an area of research known as ‘gas-phase structural biology’.

The new conference is called Advancing Mass Spectrometry for Biophysics and Structural Biology (or AMS for short), and featured 47 oral presentations and 69 posters contributed from an international group of 116 scientists representing 9 different countries.

Brandon Ruotolo, Associate Professor in the UM Chemistry Department, served as conference organizer and chair for the 2017 meeting.

The first AMS meeting covered a range of topics concerned with the development of new mass spectrometry tools capable of rapidly capturing the 3D structures and stabilities of large biomolecules present within complex mixtures at low levels.

Such technologies are of growing importance to the biopharmaceutical industry, a sector that is producing an ever increasing number of therapeutics based on large protein vehicles, an endeavor that currently represents a $60 Billion research investment in that industry.

The 2019 AMS meeting will take place in Amherst MA, at the University of Massachusetts.

More information about the meeting can be found here.