• Congratulations to Addison… Again!
    by valenra

    Congratulations to Addison for her new publication, titled “Collision Induced Unfolding Predicts the Aggregation of Glycoengineered Monoclonal Antibodies.” Sweet!

    A diagram showing how an IgG1 aggregation prediction on day 0, based on ion mobility data, correlates with the percentage of aggregation measured on day 4 in an Addison achievement.

    In this work, we developed a predictive analytical assay to assess low-pH-induced aggregation propensity in monoclonal antibodies using ion mobility-mass spectrometry and collision induced unfolding. We benchmarked our method against orthogonal methods (SEC-UV, DLS), and we investigated glycoform-specific contributions to aggregation propensity.

    Please read our publication here!

  • Congratulations to Addison!
    by valenra

    Check out the newest paper from our group looking at hidden protein conformers using ion mobility-collision induced unfolding (IM-CIU), published in JASMS!

    A magician in a top hat stands behind an Addison celebration table on a stage, holding a saw near two charts showing colored bell curves.

  • New SusCD publication!
    by valenra

    Congratulations to alumni Dr. Nicole Rivera-Fuentes and all other co-authors! In collaboration with the laboratory of Dr. Nicole Koropatkin, we investigated how the SusCD complex transports maltooligosaccharides in the gut bacterium Bacteroides thetaiotaomicron coupling Cryo-EM with native MS. This work was published in the Journal of Biological Chemistry, please check it out!

  • ASMS 2026!
    by valenra
    A group of people pose and smile around a long dinner table at an outdoor rooftop restaurant at night, with purple lighting, hanging plants above, and city buildings in the background.
  • New publication!
    by valenra

    Check the newest publication from our lab using IM-MS to look at antibody drug conjugate structure and stabilities! This work was in collaboration with Merck, co.! Check out the manuscript in Bioconjugate Chemistry! https://pubs.acs.org/doi/full/10.1021/acs.bioconjchem.6c00018

  • Congratulations to Saachi!
    by valenra

    Congratulations to Saachi for passing her Gateway exam with flashing colors! We are so proud of you and can’t wait to see what you accomplish in the future!

    A woman in a black suit and white shoes stands indoors, smiling and holding a tray of muffins. In the background, people sit and chat in a casual, brightly lit room with large windows.
  • Thomas presented at URS
    by valenra
    Thomas, wearing glasses and a name badge, stands beside his scientific research poster at an indoor URS event. The poster features graphs, charts, and text, with a University of Michigan logo at the top left corner.

    Thomas presented his work on glycans and mAb stability at the Undergraduate Research Symposium last Friday. Way to go Thomas!

  • New tutorials!
    by valenra

    Prof. Brandon Ruotolo created instructional/tutorial videos covering the basics of mass spectrometry in collab w/ Henry Stewart Talks! MC questions and learning objectives added soon to package! Please note that a subscription is needed to access these videos.

    Video 1: https://hstalks.com/t/9562/introduction-to-mass-spectrometry-1/?biosci

    Video 2: https://hstalks.com/t/16228/introduction-to-mass-spectrometry-2/?biosci

  • ISIMS 2026
    by valenra

    Our group is helping organize ISIMS in Detroit this year! Please refer to the ISIMS website for more information.

  • Congratulations to Nicole!
    by valenra

    Last week we celebrated Dr. Nicole Rivera Fuentes successfully defending her thesis in January!! The 24th graduate of the Ruotolo lab!! We wish you the best at Merck! 🍾🍾🥳🥳

CONTACT

Ruotolo Group
University of Michigan
Department of Chemistry
Room 4550
930 N. University Ave, Ann Arbor, MI 48109-1055

Phone: (+1) 734-763-2443
Fax: (1+) 734-615-3718

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