Modeling Macromolecular Assemblies

Overview

Structural biology is ultimately concerned with determining high resolution structures of all the functional macromolecules within living cells and tissues. While structural information can be obtained using a wide array of technologies, conventional methods face significant challenges when applied to dynamic, heterogeneous multi-protein targets. Consequently, there is a need to investigate new approaches that define the subunit stoichiometry, composition, and shape of heterogeneous macromolecular complexes of biological importance. In our group, we are developing ion mobility-mass spectrometry (IM-MS) based techniques to answer important structural questions about these large macromolecular complexes. Our efforts focused in structural characterization of protein complexes fall into three main categories:

  • Understanding the structural properties of proteins in the gas-phase
  • Using IM-MS measurements to develop structural models of protein complexes
  • Developing computational platforms for integrating IM-MS and other datasets into 3D models
Modelingoverview
IM-MS to model macromolecular complexes