Charge Manipulation

Many reports have indicated that the overall charge of the ions observed in nano-ESI experiments can have a significant effect on the information content of the IM-MS dataset obtained. For example, it has been observed that the quaternary structure of protein complexes is altered from its native form in more highly-charged ions in some cases. Also, evidence from collision induced dissociation (CID) has recently revealed dramatic charge-dependent mechanisms for product ion generation. The utility associated with charge manipulation is clear and we are in the process of critically evaluating a number of charge reduction approaches adapted for IM-MS analysis of protein complexes.

chargemanipulation
Different methods of charge reduction provides different gas-phase structures for multi-protein complexes

For more details, please refer to Bornschein, R. E., et al. Analyst., 2015, 140 (20).

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