Collision induced unfolding (CIU), which is a collisional activation method in the gas phase, provides a way of differentianting proteins of similar structure as well as measure the stability of proteins. CIU has been used for a variety of applications such as differentiating binding modes of different ligands, distinguishing isocross-sectional proteins, etc. CIUSuite is a software package that rapidly processes, analyzes, compares, and classifies CIU data.
For more information, please refer to Eschweiler, J. D. et al, Anal. Chem., 2015, 87 (22)